Journal: The Journal of Neuroscience

Article Title: Activity-Dependent Reconnection of Adult-Born Dentate Granule Cells in a Mouse Model of Frontotemporal Dementia

doi: 10.1523/JNEUROSCI.2724-18.2019

Figure Lengend Snippet: Increased expression of markers of inhibitory synapses in the DG of TauVLW mice and patients with FTD. A–C, Analysis of the expression of the inhibitory synapse presynaptic marker GAD65 in the DG of WT and TauVLW mice. A, GAD65+ area in different subregions of the DG in WT and TauVLW mice. In B and C, representative images of GAD65 staining in WT (B) and TauVLW (C) mice are shown. D–F, Analysis of the inhibitory synapse postsynaptic marker Gephyrin in the DG of WT and TauVLW mice. D, Gephyrin+ area in different subregions of the DG. In E and F, representative images of Gephyrin staining in WT (E) and TauVLW (F) mice are shown. In A and D, n = 10 4-month-old female mice per genotype. Five stacks of images per animal were analyzed. Asterisks indicate changes with respect to WT animals. G–I, Analysis of the expression of the inhibitory synapse presynaptic marker GAD65 in the DG of control subjects and FTD patients. G, GAD65+ area in different subregions of the DG in control subjects and FTD patients. In H and I, representative images of GAD65 staining in control subjects (H) and FTD patients (I) are shown. J–L, Analysis of the inhibitory synapse postsynaptic marker Gephyrin in the DG of control subjects and FTD patients. J, Gephyrin+ area in different subregions of the DG in control subjects and FTD patients. In K and L, representative images of Gephyrin staining in control subjects (K) and FTD patients (L) are shown. In G and J, n = 5 control subjects and n = 3 FTD patients. Five stacks of images per subject were analyzed. Asterisks indicate changes with respect to control subjects. GCL, granule cell layer; EML, External molecular layer; MML, Medial molecular layer, IML, Inner molecular layer. Yellow scale bar, 50 μm. +0.1 > p ≥ 0.05; *0.05 > p ≥ 0.01; **0.01 > p > 0.001.

Article Snippet: Dual or triple immunohistochemistry was then performed as described previously ( Llorens-Martín et al., 2013 ) using the following primary antibodies: rabbit anti-GFP (Thermo Fisher Scientific, catalog #A-11122, RRID: AB_221569 ; 1:1000); rat anti-mCherry (Thermo Fisher Scientific, catalog # {"type":"entrez-nucleotide","attrs":{"text":"M11217","term_id":"333925"}} M11217 , RRID: AB_2536611 ; 1:2500); mouse anti-IdU (BD Biosciences, catalog #347580, RRID: AB_10015219 ; 1:500); rabbit anti-Fractin (Acris Antibodies, catalog #AP08647SU-N, RRID: AB_1975531 ; 1:500); goat anti-Doublecortin (DCX) (Santa Cruz Biotechnology, catalog #sc-8066, RRID: AB_2088494 ; 1:500); mouse anti glutamic acid decarboxylase 65 (GAD65) (Developmental Hybridoma Bank, catalog #GAD-6, RRID: AB_2314498 ; 1:500); mouse anti-Gephyrin (Synaptic Systems, catalog #147 111, RRID: AB_887719 ; 1:500); rabbit anti-phospho-Tau (AT180: Thr231; 1:100) (Thermo Fisher Scientific, catalog #MN1040, RRID: AB_223649 ; 1:100); and rabbit anti-phospho-Tau (AT100: Thr212, Ser214; 1:100) (Thermo Fisher Scientific, catalog #MN1060, RRID: AB_223652 ; 1:100); guinea pig anti-cfos (Synaptic Systems, catalog #226003, RRID: AB_2231974 ; 1:500); rabbit-anti Arc (Synaptic Systems, catalog #156003, RRID: AB_887694 ; 1:1000); rat anti egr-1 (R&D Systems, catalog #MAB2818, RRID: AB_2097028 ; 1:100); mouse anti-PV (Synaptic Systems, catalog #19-5011, RRID: AB_2619882 ; 1:500); and guinea pig anti-NPY (Synaptic Systems, catalog #394004, RRID: AB_2721083 ; 1:500).

Techniques: Expressing, Marker, Staining